Contribute to Pathodopsis? - contact us
Pathodopsis is fun!
If you would like to participate in establishing the germplasm collection, below is a short protocol
describing how to spot and harvest HpA-infected plants, and what metadata are particularly useful for us.
Draba leaves are usually more pointy than those of Arabidopsis (A)
Horseweed leaves appear more 'hairy' and are often lobed (A=Arabidopsis)
Leaves of shepherd's purse are usually lobed or serrated (A=Arabidopsis)
HpA sporangiophores primarily grow out of stomata on rosette leaves, so it is the underside of
leaves that need close inspection. Infected leaves are often unhappy, and have tell-tale yellow
margins. In case plants are already flowering, stems and siliques are also common locations of
To avoid the unnatural spread of disease, tools such as tweezers should be sterilized between
touching plants – e.g. with alcohol wipes.
Another common oomycete pathogen of A. thaliana is Albugo sp. While HpA
forms a rather uniform layer of ‘powder’ on the underside of a leaf, round pustules are observed
for Albugo. Mixed infections are not uncommon.
Before harvesting plants, please ensure that the A. thaliana population at the site you are
visiting is large enough to survive even when some individuals are removed. If yes: harvesting
more than 1-2 plants per site is not needed.
Also make sure that you are not in a protected area, where sampling plants requires special
Use a spoon or tweezers to remove the entire plant from the soil, including roots and some
attached soil. Place the plant in a sufficiently large container, e.g., a 50 ml Falcon tube.
Together with plants, we need:
Keep plants in the cold (in the fridge, not in the freezer) until shipping.
Send us an email, including the picture(s) you took of the infected plant(s):
If you work at a University, we can easily arrange a Fedex pickup.
If you don’t, we will find out how else to organize transport.
Thank you for your help!!!
Contact us for more information – we can share additional pictures and metadata not displayed
on the website. firstname.lastname@example.org
Head of project:
Detlef Weigel: email@example.com